Anti-IL-1 beta/IL1B Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00101
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate,. Lane 2: rat spleen tissue lysate,. Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
规格: 100 ug/vial
价格: 315美元
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抗IL1乙/IL1B抗体
武汉博士德生物工程有限公司
目录: PA1351
domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹

Anti-IL-1 beta antibody, PA1351, Western blotting. Lane 1: Recombinant Mouse IL-1 beta Protein 10ng. Lane 2: Recombinant Mouse IL-1 beta Protein 5ng. Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng
规格: 100μg/vial
价格: 315美元
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抗IL1乙/IL1B抗体
武汉博士德生物工程有限公司
目录: PA1533
domestic rabbit 多克隆
反应物种: 大鼠
应用: 免疫印迹, 酶联免疫吸附测定

Anti- IL-1 beta antibody, PA1533, Western blotting. All lanes: Anti IL-1 beta (PA1533) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: Rat Thymus Tissue Lysate at 50ug. Predicted bind size: 31KD. Observed bind size: 31KD.
规格: 100μg/vial
价格: 315美元
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Anti-IL1 beta/IL1B Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9025
domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定

Anti-IL1 beta Picoband antibody, PB9025-1.jpg. All lanes: Anti-IL-1 beta(PB9025) at 0.5ug/ml. WB: Rat Testis Tissue Lysate at 40ug. Predicted bind size: 31KD. Observed bind size: 35KD.
规格: 100μg/vial
价格: 315美元
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Anti-Bid Il1b Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9027
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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Anti-IL1 beta/IL1B Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9606
domestic rabbit 多克隆
反应物种: 大鼠
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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Anti-IL1 beta/Il1b Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00101-1
domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹

Figure 1. Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # A00101-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 31KD. The expected band size for IL1 beta is at 31KD.
规格: 100 ug/vial
价格: 315美元
至产商