抗一氧化氮合酶, 大脑(1-181)NOS1抗体(单克隆, NOS-B1)
武汉博士德生物工程有限公司
目录: MA1072
小鼠 单克隆 (NOS-B1)
反应物种: 人类, 大鼠
应用: 免疫印迹

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M01070
domestic rabbit 单克隆 (CEI-14)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of nNOS expression in Mouse brain cell lysate (M01070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS1 monoclonal antibody (Catalog # M01070) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS1

Immunohistochemical analysis of paraffin-embedded mouse kidney, using nNOS (neuronal) Antibody(M01070). NOS1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOS1 Antibody (M01070)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
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抗磷酸化NOS1(S852)抗体
武汉博士德生物工程有限公司
目录: A01070S852
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 380美元
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抗NOS1(Y846)抗体
武汉博士德生物工程有限公司
目录: A01070Y846
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 512美元
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