抗酪氨酸羟化酶抗体(单克隆, TH-16)
武汉博士德生物工程有限公司
目录: MA1100
小鼠 单克隆 (TH-16)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化

Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100). Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, . Lane 2: mouse brain tissue lysate, . Lane 3: human U-87MG cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
规格: 100μg/vial
价格: 315美元
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Anti-Tyrosine Hydroxylase/TH Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9449
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Anti- Tyrosine Hydroxylase Picoband antibody, PB9449, Western blotting. All lanes: Anti Tyrosine Hydroxylase (PB9449) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Predicted bind size: 59KD. Observed bind size: 59KD

Anti- Tyrosine Hydroxylase Picoband antibody, PB9449,IHC(P). IHC(P): Mouse Brain Tissue

Anti- TH Picoband antibody, PB9449,IHC(P). IHC(P): Rat Brain Tissue
规格: 100μg/vial
价格: 315美元
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抗酪氨酸羟化酶TH兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01917
domestic rabbit 单克隆 (GOO-20)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Western blot analysis of Tyrosine Hydroxylase expression in PC12 cell lysate (M01917). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH monoclonal antibody (Catalog # M01917) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TH
规格: 100微升
价格: 315美元
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抗酪氨酸羟化酶TH兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01917-1
domestic rabbit 单克隆 (ECA-20)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human kidney, using Tyrosine Hydroxylase Antibody (M01917-1). TH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TH Antibody (M01917-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Tyrosine Hydroxylase expression in PC-3 cell lysate (M01917-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH monoclonal antibody (Catalog # M01917-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TH
规格: 100微升
价格: 315美元
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抗TH(磷酸化S8)抗体
武汉博士德生物工程有限公司
目录: A01917S8
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 547美元
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抗磷酸化酪氨酸羟化酶(Ser31)抗体
武汉博士德生物工程有限公司
目录: P01917
domestic rabbit 多克隆 (polyclonal)
反应物种: 大鼠
应用: 免疫印迹, 免疫细胞化学
规格: 100µl
价格: 435美元
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抗磷酸化酪氨酸羟化酶(Ser40)抗体
武汉博士德生物工程有限公司
目录: P01917-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100µl
价格: 435美元
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