Anti-Interferon gamma/IFNG Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9024
domestic rabbit 多克隆
反应物种: 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
Anti-Interferon gamma Ifng Antibody Picoband™ (monoclonal, 8E9)
武汉博士德生物工程有限公司
目录: M00393-2
小鼠 单克隆 (8000000000)
反应物种: 大鼠
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 100µg/vial
价格: 315美元
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Anti-Interferon gamma Ifng Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00393-1
domestic rabbit 多克隆
反应物种: 大鼠
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 100µg/vial
价格: 315美元
至产商
Anti-Interferon gamma/IFNG Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00393-3
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片

Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human HL-60 whole cell lysates,. Lane 5: human U-937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 2. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat lung tissue lysates,. Lane 3: mouse brain tissue lysates,. Lane 4: mouse ovary tissue lysates,. Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 3. IHC analysis of IFNG using anti-IFNG antibody (A00393-3). IFNG was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
Anti-Interferon gamma Biotinylated Ifng Antibody
武汉博士德生物工程有限公司
目录: A00393-Biotin
domestic rabbit 多克隆
反应物种: 大鼠
共轭标签: 生物素
应用: 酶联免疫吸附测定

Boster Kit Box
规格: 50µg/vial
价格: 315美元
至产商
抗IFN丙(Q69)IFNG抗体
武汉博士德生物工程有限公司
目录: A00393
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀
规格: 100微升
价格: 512美元
至产商