Anti-GAPDH Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00227
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Western blot analysis of GAPDH expression in COLO320 whole cell lysates (lane 1) and A549 whole cell lysates (lane 2). GAPDH at 36KD was detected using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog #A00227) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100μg/vial
价格: 315美元
至产商
Anti-GAPDH Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00227-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of GAPDH expression in rat brain extract (lane 1), rat kidney extract (lane 2), mouse kidney extract (lane 3), HELA whole cell lysates (lane 4) and MCF-7 whole cell lysates(lane 5). GAPDH at 36KD was detected using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog #A00227-1) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

GAPDH was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # A00227-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

GAPDH was detected in paraffin-embedded sections of human thyroid cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # A00227-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
规格: 100μg/vial
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶兔单克隆抗体, HRP共轭标签
武汉博士德生物工程有限公司
目录: H00227
domestic rabbit 单克隆 (A-7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw2647 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate with GAPDH Antibody (H00227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH monoclonal antibody (Catalog # H00227) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH
规格: 100微升
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00227-1
domestic rabbit 单克隆 (BG-7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody(M00227-1). GAPDH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GAPDH Antibody (M00227-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody (M00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH monoclonal antibody (Catalog # M00227-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH
规格: 100微升
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00227
domestic rabbit 单克隆 (BH-7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Immunofluorescent analysis of Hela cells, using GAPDH Antibody

Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody (M00227). GAPDH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GAPDH Antibody (M00227)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 2647 cell lysate; (6) Rat brain lysate with GAPDH Antibody (M00227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH monoclonal antibody (Catalog # M00227) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH
规格: 100微升
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶抗体(C末端R248)
武汉博士德生物工程有限公司
目录: A00227-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 80微升
价格: 170美元
至产商
Anti-GAPDH Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00227-4
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100 µg/vial
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶抗体(生物素)
武汉博士德生物工程有限公司
目录: A00227-Biotin
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
至产商
抗磷酸甘油醛脱氢酶抗体(HRP)
武汉博士德生物工程有限公司
目录: A00227-HRP
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
至产商
抗磷酸甘油醛脱氢酶抗体
武汉博士德生物工程有限公司
目录: M00227-2
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
规格: 50微升
价格: 170美元
至产商
抗磷酸甘油醛脱氢酶兔单克隆抗体, Clone#RM114
武汉博士德生物工程有限公司
目录: M00227-5
domestic rabbit 单克隆 (RM114)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀
规格: 100微升
价格: 399美元
至产商
抗磷酸甘油醛脱氢酶抗体(单克隆, 磷酸甘油醛脱氢酶-71.1)
武汉博士德生物工程有限公司
目录: M00227-6
小鼠 单克隆 (GAPDH-71.1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100µl/vial
价格: 240美元
至产商
抗磷酸甘油醛脱氢酶小鼠单克隆抗体
武汉博士德生物工程有限公司
目录: M00227-7
小鼠 单克隆 (17G14)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
规格: 100 µl/vial
价格: 315美元
至产商
抗磷酸甘油醛脱氢酶单克隆抗体[12D3D5](生物素)
武汉博士德生物工程有限公司
目录: M00227-Biotin
小鼠 单克隆 (12D3D5)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
至产商