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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 Hsp90aa1 抗体
Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9089
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Anti-Hsp90-alpha-Picoband antibody, PB9089-1.JPG. IHC(P): Human Intestinal Cancer Tissue
Anti-Hsp90-alpha-Picoband antibody, PB9089-2.JPG. IHC(P): Mouse Brain Tissue
Anti-Hsp90-alpha-Picoband antibody, PB9089-3.JPG. IHC(P): Rat Brain Tissue
规格: 100μg/vial
价格: 315美元
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Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9635
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Anti- Hsp90 alpha Picoband antibody, PB9635, Western blotting. All lanes: Anti Hsp90 alpha (PB9635) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: 22RV1 Whole Cell Lysate at 40ug. Predicted bind size: 90KD. Observed bind size: 90KD
Anti- Hsp90 alpha Picoband antibody, PB9635, IHC(P). IHC(P): Mouse Testis Tissue
Anti- Hsp90 alpha Picoband antibody, PB9635, IHC(P). IHC(P): Rat Testis Tissue
规格: 100μg/vial
价格: 315美元
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抗Hsp90甲+乙HSP90AA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01103-2
domestic rabbit 单克隆 (EBD-8)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
免疫沉淀
Western blot analysis of Hsp90 alpha + beta expression in HEK-293 cell lysate (M01103-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Hsp90 alpha + beta Antibody (M01103-2). HSP90AA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AA1 Antibody (M01103-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
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抗Hsp90甲HSP90AA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01103-1
domestic rabbit 单克隆 (CCF-8)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsp90 alpha antibody(M01103-1). HSP90AA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AA1 Antibody (M01103-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis on HeLa cell lysate using HSP90A Antibody (M01103-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
规格: 100微升
价格: 315美元
至产商
抗Hsp90甲HSP90AA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01103-3
domestic rabbit 单克隆 (BAC-8)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
Western blot analysis of Hsp90 alpha expression in (1)PC-12 cell lysate; (2)HeLa cell lysate (M01103-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-3) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
规格: 100微升
价格: 315美元
至产商
抗Hsp90甲HSP90AA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01103
domestic rabbit 单克隆 (BAD-8)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
Western blot analysis of Hsp90 alpha expression in Jurkat cell lysate (M01103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
规格: 100微升
价格: 315美元
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