Anti-DC-SIGN/CD209 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01025-2
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of DC-SIGN using anti-DC-SIGN antibody (A01025-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human MCF-7 whole cell lysates,. Lane 3: human HepG2 whole cell lysates,. Lane 4: human A549 whole cell lysates,. Lane 5: rat spleen tissue lysates,. Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DC-SIGN antigen affinity purified polyclonal antibody (Catalog # A01025-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DC-SIGN at approximately 46KD. The expected band size for DC-SIGN is at 46KD.

Figure 2. IHC analysis of DC-SIGN using anti-DC-SIGN antibody (A01025-2). DC-SIGN was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DC-SIGN Antibody (A01025-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of DC-SIGN using anti-DC-SIGN antibody (A01025-2). DC-SIGN was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DC-SIGN Antibody (A01025-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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抗人类直流-SIGN DyLight系列染料®488共轭标签CD209抗体
武汉博士德生物工程有限公司
目录: A01025-Dyl488
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗人类直流-SIGN DyLight系列染料®550共轭标签CD209抗体
武汉博士德生物工程有限公司
目录: A01025-Dyl550
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗直流-SIGN CD209单克隆抗体[5D7]
武汉博士德生物工程有限公司
目录: M01025-1
小鼠 单克隆 (5D7)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 0.1毫克
价格: 404美元
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Anti-Hu CD209 Purified Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M01025-2
小鼠 单克隆 (UW60.1)
反应物种: 人类
应用: 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 0.1毫克(C100)
价格: 280美元
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抗CD209兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01025-3
domestic rabbit 单克隆 (17C54)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
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