Anti-HIF-1-alpha/HIF1A Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9253
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of HIF-1-alpha/HIF1A using anti-HIF-1-alpha/HIF1A antibody (PB9253). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, . Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-1-alpha/HIF1A antigen affinity purified polyclonal antibody (Catalog # PB9253) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-1-alpha/HIF1A at approximately 115-120KD. The expected band size for HIF-1-alpha/HIF1A is at 97KD.

Figure 2. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). HIF 1 alpha was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). HIF 1 alpha was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
Anti-HIF-1 alpha/HIF1A Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00013-1
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100µg/vial
价格: 315美元
至产商
Anti-HIF-1 alpha/HIF1A Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00013-3
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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抗HIF-1甲(缺氧诱导因子1A)兔单克隆抗体, Clone#RM242
武汉博士德生物工程有限公司
目录: M00013-1
domestic rabbit 单克隆 (RM242)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 399美元
至产商
抗HIF甲缺氧诱导因子1A单克隆抗体
武汉博士德生物工程有限公司
目录: M00013-2
小鼠 单克隆 (1A3)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 380美元
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抗HIF-1甲兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00013-3
domestic rabbit 单克隆 (17H18)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
规格: 100 µl/vial
价格: 315美元
至产商