抗HNF-4兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00389
domestic rabbit 单克隆 (GBA-8)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Western blot analysis of HNF-4-alpha expression in A549 cell lysate (M00389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A monoclonal antibody (Catalog # M00389) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF4A
规格: 100微升
价格: 315美元
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抗HNF4甲/丙(K127)HNF4A抗体
武汉博士德生物工程有限公司
目录: A00389
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 512美元
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抗HNF4甲(磷酸化S313)HNF4A抗体
武汉博士德生物工程有限公司
目录: A00389S313
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫组化
规格: 100微升
价格: 547美元
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抗磷酸化HNF4(S313)抗体
武汉博士德生物工程有限公司
目录: A00389S313-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 380美元
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Anti-HNF-4-alpha Antibody Picoband™ (monoclonal, 6C8E9)
武汉博士德生物工程有限公司
目录: M00389-2
小鼠 单克隆 (6C8E9)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片
规格: 100 µg/vial
价格: 315美元
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