抗Hsp70/HSPA1A/HSPA1B抗体
武汉博士德生物工程有限公司
目录: PA1214
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Figure 1. Western blot analysis of Hsp70 using anti- Hsp70 antibody (PA1214). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, . Lane 2: human Caco-2 whole cell lysates, . Lane 3: human PC-3 whole cell lysates, . Lane 4: human THP-1 whole cell lysates, . Lane 5: human U2OS whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Hsp70 antigen affinity purified polyclonal antibody (Catalog # PA1214) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp70 at approximately 70KD. The expected band size for Hsp70 is at 70KD.

Figure 2. IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
抗Hsp70 HSPA1A兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00949
domestic rabbit 单克隆 (DGE-8)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody (M00949). HSPA1A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA1A Antibody (M00949)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of HSP70 expression in (1) K562 cell lysate; (2) 293T cell lysate (M00949). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1A monoclonal antibody (Catalog # M00949) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA1A
规格: 100微升
价格: 315美元
至产商
Anti-Hsp70 HSPA1A Antibody Picoband™ (monoclonal, 3H5)
武汉博士德生物工程有限公司
目录: M00949-2
小鼠 单克隆 (3H5)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. IHC analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). Hsp70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-Hsp70 Antibody (M00949-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. Western blot analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,. Lane 2: human COLO-320 whole cell lysate,. Lane 3: human SW620 whole cell lysate,. Lane 4: human A431 whole cell lysate,. Lane 5: human A549 whole cell lysate,. Lane 6: human HepG2 whole cell lysate,. Lane 7: human PANC-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hsp70 antigen affinity purified monoclonal antibody (Catalog # M00949-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
规格: 100µg/vial
价格: 315美元
至产商
抗人类Hsp70 DyLight系列染料®488共轭标签HSPA1A抗体(单克隆, 3H5)
武汉博士德生物工程有限公司
目录: M00949-Dyl488
小鼠 单克隆 (3H5)
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
至产商
抗人类Hsp70 DyLight系列染料®550共轭标签HSPA1A抗体(单克隆, 3H5)
武汉博士德生物工程有限公司
目录: M00949-Dyl550
小鼠 单克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
至产商
抗HSP70单克隆抗体
武汉博士德生物工程有限公司
目录: M00949-1
小鼠 单克隆 (3A3)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀
规格: 100微克
价格: 357美元
至产商