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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 ICAM-1 抗体
Anti-ICAM1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9004
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
Anti-ICAM1 Picoband antibody, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Anti-ICAM1 Picoband antibody,IHC(P) . IHC(P): Human Lung Cancer Tissue
Anti-ICAM1 Picoband antibody, Western blotting. All lanes: Anti-ICAM1(PB9004) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD
规格: 100μg/vial
价格: 315美元
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Anti-ICAM1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9017
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
,
免疫组化-冰冻切片
Anti-ICAM1 Picoband antibody, PB9017-1.JPG. IHC(P): Human Intestinal Cancer Tissue
Anti-ICAM1 Picoband antibody, PB9017-2.jpg. All lanes: Anti-ICAM1(PB9017) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD
Figure 3. IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) . ICAM1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100μg/vial
价格: 315美元
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Anti-ICAM1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00171
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,. Lane 2: rat thymus tissue lysate,. Lane 3: rat RH35 cell lysate,. Lane 4: mouse spleen tissue lysate,. Lane 5: mouse thymus tissue lysate,. Lane 6: mouse HEPA1-6 cell lysate,. Lane 7: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
规格: 100µg/vial
价格: 315美元
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Anti-Human CD54 Icam1 Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00171-FITC-1
小鼠 单克隆 (LB-2)
反应物种:
人类
共轭标签: 异硫氰酸荧光素
应用:
流式细胞仪
Daudi gated cells stained with FITC conjugated mouse anti-human CD54 (clone LB-2) (red histogram). Daudi gated cells stained with FITC conjugated mouse IgG2b isotype control (Blue histogram).
规格: 100试验, 200试验
价格: 235美元
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抗细胞间粘附分子1/Cd54兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00171-1
domestic rabbit 单克隆 (DOD-9)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
Western blot analysis of ICAM1 expression in Raji cell lysate (M00171-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 monoclonal antibody (Catalog # M00171-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1
规格: 100微升
价格: 315美元
至产商
Anti-ICAM1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00171-2
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
规格: 100 µg/vial
价格: 315美元
至产商
抗磷酸化ICAM(Y512)抗体
武汉博士德生物工程有限公司
目录: A00171Y512-1
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
规格: 100微升
价格: 380美元
至产商
抗CD54/ICAM-1单克隆抗体
武汉博士德生物工程有限公司
目录: M00171
小鼠 单克隆 (W-CAM-1; same as Wehi-CAM-1 or 1H4)
反应物种:
人类
应用:
免疫组化
规格: 100 ug/vial
价格: 440美元
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Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00171-2
小鼠 单克隆 (MEM-111)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格: 0.1毫克(C100)
价格: 280美元
至产商
Anti-ICAM1 Antibody Picoband™ (monoclonal, 6F2C3)
武汉博士德生物工程有限公司
目录: M00171-3
小鼠 单克隆 (6F2C3)
反应物种:
人类
应用:
免疫印迹
,
流式细胞仪
,
免疫组化-石蜡切片
规格: 100 µg/vial
价格: 315美元
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