domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹
反应物种: 人类, 大鼠
应用: 免疫印迹
Anti-IGFBP-3 antibody, PA1498, Western blotting. All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml. Lane 1: 293T Whole Cell Lysate at 40ug. Lane 2: MCF- 7 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 32KD. Observed bind size: 40KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
Figure 1. Western blot analysis of IGFBP-3 using anti-IGFBP-3 antibody (PB9710). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, . Lane 2: rat brain tissue lysates, . Lane 3: rat liver tissue lysates, . Lane 4: rat PC-12 whole cell lysates. Lane 5: human U-87MG whole cell lysates. Lane 6: mouse kidney tissue lysates, . Lane 7: mouse heart tissue lysates, . Lane 8: mouse brain tissue lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-3 antigen affinity purified polyclonal antibody (Catalog # PB9710) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP-3 at approximately 40KD. The expected band size for IGFBP-3 is at 32KD.
Figure 2. IHC analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710). IGFBP3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGFBP3 Antibody (PB9710) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定
Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.
规格: 100 ug/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
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