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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 IL-1beta 抗体
Anti-IL-1 beta/IL1B Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00101
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate,. Lane 2: rat spleen tissue lysate,. Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
规格: 100 ug/vial
价格: 315美元
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抗IL1乙/IL1B抗体
武汉博士德生物工程有限公司
目录: PA1341
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Anti-IL-1 beta antibody, PA1341, Western blotting. Lane 1: Recombinant Human IL-1 beta Protein 20ng. Lane 2: Recombinant Human IL-1 beta Protein 10ng
规格: 100μg/vial
价格: 315美元
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Anti-Bid Il1b Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9027
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Figure 1. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of BID using anti-BID antibody (PB9027). BID was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗IL1乙兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00101-1
domestic rabbit 单克隆 (BII-9)
反应物种:
人类
应用:
免疫印迹
Western blot analysis of extracts from recombinant IL1 beta, using IL1 beta antibody (M00101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B monoclonal antibody (Catalog # M00101-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1B
规格: 100微升
价格: 315美元
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抗IL1乙兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00101
domestic rabbit 单克隆 (IBF-9)
反应物种:
人类
应用:
免疫印迹
Western blot analysis of IL1 beta expression in recombinant IL1 beta protein (M00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B monoclonal antibody (Catalog # M00101) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1B
规格: 100微升
价格: 315美元
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抗IL-1乙抗体
武汉博士德生物工程有限公司
目录: A00101-2
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
规格: 1毫克
价格: 1274美元
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