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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 IL-4 抗体
抗白细胞介素4白细胞介素-4抗体
武汉博士德生物工程有限公司
目录: PA1424
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Anti-IL-4 antibody, PA1424, Western blotting. Lane 1: Recombinant Human IL-4 Protein 10ng. Lane 2: Recombinant Human IL-4 Protein 5ng. Lane 3: Recombinant Human IL-4 Protein 2.5ng
规格: 100μg/vial
价格: 315美元
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抗白细胞介素4白细胞介素-4抗体
武汉博士德生物工程有限公司
目录: PA1749
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
Anti-IL-4 antibody, PA1749, Western blotting. All lanes: Anti IL-4 (PA1749) at 0.5ug/ml. Lane 1: Recombinant Human IL-4 Protein 10ng. Lane 2: Recombinant Human IL-4 Protein 5ng. Lane 3: Recombinant Human IL-4 Protein 2.5ng. Predicted bind size: 14KD. Observed bind size: 14KD
Anti-IL-4 antibody, PA1749, IHC(P). IHC(P): Human Tonsil Tissue
规格: 100μg/vial
价格: 315美元
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抗白细胞介素4白细胞介素-4抗体
武汉博士德生物工程有限公司
目录: RP1009
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
Figure. Western blot analysis of IL-4 using anti- IL-4 antibody (RP1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IL-4 Protein 0.5ng,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-4 antigen affinity purified polyclonal antibody (Catalog # RP1009) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 14KD. The expected band size for IL-4 is at 14KD.
规格: 100μg/vial
价格: 315美元
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抗白细胞介素-4兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00230
domestic rabbit 单克隆 (AADD-9)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
Western blot analysis of IL4 expression in human tonsil lysate (M00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 monoclonal antibody (Catalog # M00230) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4
规格: 100微升
价格: 315美元
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Anti-IL4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00230
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
Figure 1. Western blot analysis of IL4 using anti-IL4 antibody (A00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (Catalog # A00230) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4 at approximately 20KD. The expected band size for IL4 is at 17KD.
规格: 100µg/vial
价格: 315美元
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抗IL-4抗体
武汉博士德生物工程有限公司
目录: A00230-2
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
规格: 100微升
价格: 512美元
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