Anti-IL8/CXCL8 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9621
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片

Figure 1. Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 34KD. The expected band size for IL-8 is at 11KD.

Figure 2. IHC analysis of IL-8 using anti-IL-8 antibody (PB9621). IL-8 was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL-8 Antibody (PB9621) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗IL-8 CXCL8抗体
武汉博士德生物工程有限公司
目录: A00423
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
规格: 1毫升
价格: 1274美元
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抗IL-8 CXCL8单克隆抗体
武汉博士德生物工程有限公司
目录: A00423-1
小鼠 单克隆 (8B1)
反应物种: 人类, 小鼠
应用: 免疫组化, 免疫组化-石蜡切片
文章摘录数: 1
规格: 100µl
价格: 399美元
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抗IL-8 CXCL8单克隆抗体
武汉博士德生物工程有限公司
目录: M00423
小鼠 单克隆 (3E4D8)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 380美元
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抗白细胞介素-8兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00423-1
domestic rabbit 单克隆 (26C79)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
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