domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Western blot analysis of Integrin linked ILK expression in rat lung extract (lane 1), mouse liver extract (lane 2) and HELA whole cell lysates (lane 3). Integrin linked ILK at 55KD was detected using rabbit anti- Integrin linked ILK Antigen Affinity purified polyclonal antibody (Catalog # PB9949) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (FHE-9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
Western blot analysis of ILK expression in K562 cell lysate (M02932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK monoclonal antibody (Catalog # M02932) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILK
规格: 100微升
价格: 315美元
至产商
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫组化-石蜡切片
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
微博分享
关注我们的微博
- 来邦网
- 英文来邦