Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody
武汉博士德生物工程有限公司
目录: PA1611
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-IDO1 antibody, PA1611, Western blotting. All lanes: Anti IDO1 (PA1611) at 0.5ug/ml. Lane 1: SMMC Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Lane 3: Human Placenta Tissue Lysate at 50ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: U87 Whole Cell Lysate at 40ug. Lane 6: 293T Whole Cell Lysate at 40ug. Lane 7: A431 Whole Cell Lysate at 40ug. Lane 8: HELA Whole Cell Lysate at 40ug. Lane 9: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 45KD. Observed bind size: 45KD
规格: 100μg/vial
价格: 315美元
至产商
Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9603
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of IDO1 using anti- IDO1 antibody (PB9603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IDO1 antigen affinity purified polyclonal antibody (Catalog # PB9603) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO1 at approximately 45KD. The expected band size for IDO1 is at 45KD.

Figure 2. IHC analysis of IDO1 using anti-IDO1 antibody (PB9603). IDO1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IDO1 Antibody (PB9603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of IDO1 using anti-IDO1 antibody (PB9603). IDO1 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IDO1 Antibody (PB9603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
抗INDO IDO1抗体
武汉博士德生物工程有限公司
目录: A01705-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 512美元
至产商
Anti-IDO1/Ido Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M01705
小鼠 单克隆 (2E2.6 IgG1)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
规格: 100微克
价格: 487美元
至产商
Anti-IDO1/Ido Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M01705-1
小鼠 单克隆 (10.1 IgG3)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
规格: 100微克
价格: 487美元
至产商
抗INDO兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01705-4
domestic rabbit 单克隆 (17I51)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格: 100 µl/vial
价格: 315美元
至产商