抗CD18/ITGB2抗体
武汉博士德生物工程有限公司
目录: PA1124
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-CD18 antibody, PA1124, IHC(P). IHC(P): Human Uroepithelium Cancer Tissue

Anti-CD18 antibody, PA1124, Western blotting. All lanes: Anti CD18 (PA1124) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD
规格: 100μg/vial
价格: 315美元
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抗CD18/ITGB2抗体
武汉博士德生物工程有限公司
目录: PA1623
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Anti-CD18 antibody, PA1623, Western blotting. Lane 1: JURKAT Cell Lysate . Lane 2: CEM Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: SMMC Cell Lysate. Lane 5: HELA Cell Lysate.

Anti-CD18 antibody, PA1623, IHC(P). IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
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Anti-CD18/ITGB2 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9141
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of CD18 using anti-CD18 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CD18 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18 at approximately 40KD. The expected band size for CD18 is at 40KD.

Figure 2. Western blot analysis of CD18 using anti-CD18 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate. Lane 2: CEM Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18 at approximately 100KD. The expected band size for CD18 is at 85KD.

Figure 3. IHC analysis of CD18 using anti-CD18 antibody (PB9141). CD18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD18 Antibody (PB9141) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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Anti-CD18 ITGB2 Antibody Picoband™ (monoclonal, 1A3/2A10)
武汉博士德生物工程有限公司
目录: M00458-1
小鼠 单克隆 (1A3/2A10)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片

Figure 1. IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. Western blot analysis of CD18 using anti-CD18 antibody (M00458-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD18 antigen affinity purified monoclonal antibody (Catalog # M00458-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
规格: 100µg/vial
价格: 315美元
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抗人类CD18 DyLight系列染料®550共轭标签ITGB2抗体(单克隆, 1A3/2A10)
武汉博士德生物工程有限公司
目录: M00458-Dyl550
小鼠 单克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗CD18 ITGB2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00458
domestic rabbit 单克隆 (AAIH-9)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
规格: 100微升
价格: 315美元
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Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00458-2
小鼠 单克隆 (MEM-48)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
规格: 0.1毫克(C100)
价格: 280美元
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