Anti-Cytokeratin 8/KRT8 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01421
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody (A01421). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: mouse ovary tissue lysates, . Lane 3: mouse liver tissue lysates, . Lane 4: MCF-7 whole cell lysates,. Lane 5: A549 whole cell lysates,. Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.

Figure 2. IHC analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody (A01421). . Cytokeratin 8 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cytokeratin 8 Antibody (A01421) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody (A01421). . Cytokeratin 8 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Cytokeratin 8 Antibody (A01421) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗PCK-26 Krt8抗体(单克隆, PCK-26)
武汉博士德生物工程有限公司
目录: MA1082
小鼠 单克隆 (PCK-26)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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抗角蛋白8/KRT8抗体
武汉博士德生物工程有限公司
目录: PA1240
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
抗角蛋白8 KRT8兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01421-2
domestic rabbit 单克隆 (CIG-11)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody(M01421-2). KRT8 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT8 Antibody (M01421-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Cytokeratin 8 expression in HaCaT cell lysate (M01421-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT8 monoclonal antibody (Catalog # M01421-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT8
规格: 100微升
价格: 315美元
至产商