抗SMAD家族成员2抗体
武汉博士德生物工程有限公司
目录: PA1898
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Anti-Smad2 antibody, PA1898, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD (162aa tag+D424-P578). Lane 1: Recombinant Human SHC1 Proteins 10ng . Lane 2: Recombinant Human SHC1 Proteins 5ng . Lane 3: Recombinant Human SHC1 Proteins 2.5ng

Anti-Smad2 antibody, PA1898, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-Smad2 antibody, PA1898, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: JURKAT Cell Lysate. Lane 5: U87 Cell Lysate. Lane 6: MCF-7 Cell Lysate.
规格: 100μg/vial
价格: 315美元
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抗磷酸化SMAD家族成员2(S255)兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00090
domestic rabbit 单克隆 (HBE-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀

Western blot analysis of Phospho-Smad2 (S255) expression in Hela cell treated with Okadaic acid and Calyculin A lysate (P00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
规格: 100微升
价格: 315美元
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抗SMAD家族成员2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00090-1
domestic rabbit 单克隆 (ACI-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded rat stomach, using Smad2 Antibody(M00090-1). SMAD2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (M00090-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Smad2 expression in (1) Jurkat cell lysate;(2) RAW2647 cell lysate (M00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # M00090-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
规格: 100微升
价格: 315美元
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抗SMAD家族成员2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00090
domestic rabbit 单克隆 (EFF-19)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Western blot analysis of Smad2 expression in Jurkat cell lysate (M00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # M00090) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
规格: 100微升
价格: 315美元
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抗SMAD家族成员2抗体
武汉博士德生物工程有限公司
目录: A00090-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. Lane 2: rat liver tissue lysates. Lane 3: mouse testicular tissue lysates. Lane 4: mouse heart tissue lysates. Lane 5: mouse lung tissue lysates. Lane 6: mouse lung tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.

Figure 2. IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). SMAD2 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). SMAD2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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抗磷酸化SMAD家族成员2(S250)兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00090-1
domestic rabbit 单克隆 (HBC-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪

Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (P00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
规格: 100微升
价格: 315美元
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Anti-SMAD2 Antibody Picoband™ (monoclonal, 3C4)
武汉博士德生物工程有限公司
目录: M00090-3
小鼠 单克隆 (3C4)
反应物种: 人类
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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