兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
SMAD3 was detected in paraffin-embedded sections of epidermal keratinocytes at 40X using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 2.5 ug/mL. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Western blot analysis of endogenous SMAD3 expression in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. Phosphorylated SMAD3 was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059). Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
Western blot analysis of SMAD3 expression in HEK293 lysate (lane 1), HeLa Lysate (lane 2), MCF-7 Lysate (lane 3), Jurkat Lysate (lane 4), A431 Lysate (lane 5), A549 Lysate (lane 6), LNCap Lysate (lane 7), MOLT-4 Lysate (lane 8), Ramos Lysate (lane 9), Raji Lsyate (lane 10), A-172 Lysate (lane 11) and NIH/3T3 Lysate (lane 12). Phosphorylated SMAD3 at 35KD was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 1ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100微升
价格: 339美元
至产商
兔 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Anti- SMAD3 Picoband antibody, PB9396, Western blotting. All lanes: Anti SMAD3 (PB9396) at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: Human Placenta Tissue Lysate at 50ug. Lane 3: PANC Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 48KD. Observed bind size: 56KD
规格: 100 ug/vial
价格: 240美元
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兔 单克隆 (FG-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Western blot analysis of Smad3 expression in (1) Jurkat cell lysate; (2) Rat liver lysate (M00059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD3 monoclonal antibody (Catalog # M00059) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD3
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody(M00059). SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (M00059)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100 ug/vial
价格: 299美元
至产商
兔 单克隆 (AHO-19)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Western blot analysis of Phospho-Smad3(S423/S425) expression in PC-12 cell lysate treated with 1%BSA (P00059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD3 monoclonal antibody (Catalog # P00059-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD3
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody(P00059-1). SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (P00059-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunofluorescent analysis of A549 cells treated with TGFbeta , using Phospho-Smad3 (S423 + S425) Antibody.
规格: 100 ug/vial
价格: 299美元
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