小鼠 单克隆 (FR4D11)
反应物种: 人类
应用: 流式细胞仪
反应物种: 人类
应用: 流式细胞仪
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
小鼠 单克隆 (FR4D11)
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
小鼠 单克隆 (FR4D11)
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪
兔 单克隆 (GEH-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Western blot analysis of CD10 expression in Jurkat lysate (M04065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MME monoclonal antibody (Catalog # M04065) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MME
规格: 100 ug/vial
价格: 299美元
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