Anti-MUC2 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01212
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫细胞化学, 免疫组化-石蜡切片

Figure 1. IHC analysis of MUC2 using anti-MUC2 antibody (A01212). MUC2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of MUC2 using anti-MUC2 antibody (A01212). MUC2 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of MUC2 using anti-MUC2 antibody (A01212). MUC2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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抗MUC2(粘蛋白2)单克隆抗体
武汉博士德生物工程有限公司
目录: M01212
小鼠 单克隆 (MLP/842)
反应物种: 人类
应用: 免疫组化

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withMUC2 Monoclonal Antibody (MLP/842).
规格: 100 ug/vial
价格: 440美元
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抗MUC2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01212-1
domestic rabbit 单克隆 (AAEO-13)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of MUC2 expression in Caco-2 cell lysate (M01212-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC2 monoclonal antibody (Catalog # M01212-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC2
规格: 100微升
价格: 315美元
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抗粘蛋白2 MUC2抗体
武汉博士德生物工程有限公司
目录: A01212-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 697美元
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Anti-MUC2 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01212-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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Anti-MUC2 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01212-3
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 酶联免疫吸附测定, 免疫组化-石蜡切片

Figure 1. IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 2. IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Figure 3. IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
规格: 100 µg/vial
价格: 315美元
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