小鼠 单克隆 (45M1)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片
Anti-Mucin 5AC antibody, PA2303, All Western blotting. All lanes: Anti-MUC5AC(PA2303) at0.5ug/ml. Lane 1: SGC Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: SW620 Whole Cell Lysate at 40ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: PANC Whole Cell Lysate at 40ug. Predicted bind size: 527KD. Observed bind size: 300KD
Figure 2. IHC analysis of Mucin 5AC using anti-Mucin 5AC antibody (PA2303). Mucin 5AC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Mucin 5AC Antibody (PA2303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Anti- Mucin 5AC Picoband antibody, PB9286, Western blotting. All lanes: Anti Mucin 5AC (PB9286) at 0.5ug/ml. WB: Recombinant Human Mucin5A Protein 0.5ng. Predicted bind size: 68KD. Observed bind size: 68KD
Anti- Mucin 5AC Picoband antibody, PB9286, IHC(P). IHC(P): Human Gastric Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (ABAD-13)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Western blot analysis of MUC5AC expression in human stomach lysate (M00612-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC5AC monoclonal antibody (Catalog # M00612-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC5AC
规格: 100微升
价格: 315美元
至产商
小鼠 单克隆 (SPM297)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化
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