抗c Myc抗体(单克隆, 9E10)
武汉博士德生物工程有限公司
目录: MA1028
小鼠 单克隆 (90000000000)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
抗c Myc抗体
武汉博士德生物工程有限公司
目录: PA1555
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
抗磷酸化c(S62)兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00026-2
domestic rabbit 单克隆 (EAC-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody (P00026-2). MYC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYC Antibody (P00026-2)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Phospho-c-Myc (S62) expression in HeLa cell lysate (P00026-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC monoclonal antibody (Catalog # P00026-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYC
规格: 100微升
价格: 315美元
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Anti-c-Myc Oncoprotein Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00026
小鼠 单克隆 (SPM237)
反应物种: 人类
应用: 免疫组化
Anti-c-Myc Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9092
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
抗c Myc单克隆抗体
武汉博士德生物工程有限公司
目录: M00026-1
domestic rabbit 单克隆 (AHI-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.

Figure 2. IHC analysis of MYC using anti-MYC antibody (M00026-1)on paraffin-embedded human lung cancer. MYC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYC Antibody (M00026-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of MYC using anti-MYC antibody (M00026-1) in 1) Jurkat cell lysate;(2) K562 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody (Catalog # M00026-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MYC.
规格: 100微升
价格: 315美元
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抗Myc(R367)抗体
武汉博士德生物工程有限公司
目录: A00026
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫组化
规格: 100微升
价格: 512美元
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Anti-c-Myc/MYC Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00026-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
规格: 100 µg/vial
价格: 315美元
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抗磷酸化c(S373)抗体
武汉博士德生物工程有限公司
目录: A00026S373
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 酶联免疫吸附测定, 免疫组化, 免疫沉淀
规格: 100微升
价格: 380美元
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抗c Myc(磷酸化S62)抗体
武汉博士德生物工程有限公司
目录: A00026S62-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
规格: 100微升
价格: 697美元
至产商
抗磷酸化c(T358)抗体
武汉博士德生物工程有限公司
目录: A00026T358
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 酶联免疫吸附测定, 免疫组化, 免疫沉淀
规格: 100微升
价格: 380美元
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抗c Myc(磷酸化T58)抗体
武汉博士德生物工程有限公司
目录: A00026T58-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 697美元
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抗c Myc(磷酸化T58/S62)抗体
武汉博士德生物工程有限公司
目录: A00026T58S62
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀
规格: 100微升
价格: 697美元
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抗Myc抗体
武汉博士德生物工程有限公司
目录: M00026-3
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹
规格: 50微升
价格: 170美元
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