domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Anti-ATM antibody, PA1784, Western blotting. Lane 1: Rat Testis Tissue Lysate . Lane 2: U87 Cell Lysate . Lane 3: MCF-7 Cell Lysate .
Anti-ATM antibody, PA1784, Western blotting. Lane 1: HELA Cell Lysate . Lane 2: SMMC Cell Lysate . Lane 3: U87 Cell Lysate . Lane 4: A549 Cell Lysate . Lane 5: MCF-7 Cell Lysate .
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
domestic rabbit 单克隆 (AEE-1)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin (P00014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM monoclonal antibody (Catalog # P00014-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATM
Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody(P00014-1). ATM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATM Antibody (P00014-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
至产商
domestic rabbit 单克隆 (BCF-1)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
Immunofluorescent analysis of Hela cells, using ATM Antibody.
Figure 2. IHC analysis of ATM using anti-ATM antibody (M00014). ATM was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATM Antibody (M00014) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of ATM using anti-ATM antibody (M00014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (Catalog # M00014) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ATM.
规格: 100微升
价格: 315美元
至产商
domestic rabbit 单克隆 (AECA-1)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
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