Anti-NFkB p105/p50/NFKB1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9149
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
文章摘录数: 2

Anti-NFkB p105/P50 Picoband antibody, PB9149-1.jpg. All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml. WB: Recombinant Human NFKBP105 Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD

Anti-NFkB p105/P50 Picoband antibody, PB9149-2.jpg. All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: A431 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 50KD . Observed bind size: 50KD

Anti-NFkB p105/P50 Picoband antibody, PB9149-3.JPG. IHC(P): Human Lung Cancer Tissue
规格: 100μg/vial
价格: 315美元
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抗NFkB p105/p50/NFKB1抗体
武汉博士德生物工程有限公司
目录: PA2111
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Anti-NFkB p105/p50 antibody, PA2111, Western blotting. All lanes: Anti NFkB p105/p50 (PA2111) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Rat Spleen Tissue Lysate at 50ug. Lane 3: Rat Testis Tissue Lysate at 50ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: A431 Whole Cell Lysate at 40ug. Lane 6: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 50KD. Observed bind size: 105KD
规格: 100μg/vial
价格: 315美元
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抗核因子κB(p105/p50)NFKB1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00283
domestic rabbit 单克隆 (ID-14)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Immunohistochemical analysis of paraffin-embedded human kidney, using NF-kappaB (p105/p50) Antibody(M00283). NFKB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NFKB1 Antibody (M00283)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of NF-kappaB (p105/p50) expression in (1)PC-12 cell lysate;(2) NIH/3T3 cell lysate (M00283). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKB1 monoclonal antibody (Catalog # M00283) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFKB1
规格: 100微升
价格: 315美元
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抗NFkB p105/p50/NFKB1抗体
武汉博士德生物工程有限公司
目录: PA2111-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Anti- NFkB p105/p50 antibody, PA2111-1, Western blotting. All lanes: Anti NFkB p105/p50(PA2111-1) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 105KD. Observed bind size: 105KD
规格: 100μg/vial
价格: 315美元
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