抗β神经生长因子神经生长因子抗体
武汉博士德生物工程有限公司
目录: RP1025
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
抗神经生长因子/乙神经生长因子兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00341
domestic rabbit 单克隆 (BDE-14)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Immunohistochemical analysis of paraffin-embedded human kidney, using NGF Antibody(M00341). NGF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NGF Antibody (M00341)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of NGF expression in (1)Mouse thyroid lysate;(2) HeLa cell lysate (M00341). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF monoclonal antibody (Catalog # M00341) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF

IF analysis of immunocytochemical section of SH-SY5Y using anti- NGF antibody (M00341) . NGF was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- NGF Antibody (M00341) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
至产商
Anti-NGF Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00341-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100µg/vial
价格: 315美元
至产商
Anti-NGF Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00341-3
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100 µg/vial
价格: 315美元
至产商