Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Mon ...
武汉博士德生物工程有限公司
目录: MA1055
小鼠 单克隆 (MAPK-YT)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 1

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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抗ERK1/MAPK3抗体
武汉博士德生物工程有限公司
目录: PA1343
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Anti-ERK1 antibody, PA1343, Western blotting. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Thymus Tissue Lysate. Lane 3: Rat Skeletal Muscle Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: HELA Cell Lysate. Lane 6: JURKAT Cell Lysate. Lane 7: RAJI Cell Lysate. Lane 8: COLO320 Cell Lysate

Anti-ERK1 antibody, PA1343, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-ERK1 antibody, PA1343, IHC(P). ICC: HELA Cell
规格: 100μg/vial
价格: 315美元
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抗ERK1/2 MAPK3兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00104-1
domestic rabbit 单克隆 (DFF-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates (M00104-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
规格: 100微升
价格: 315美元
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Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) MAPK3 Rabbit Monoclonal Antibod ...
武汉博士德生物工程有限公司
目录: P00104
domestic rabbit 单克隆 (BIH-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀

Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (P00104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # P00104) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
规格: 100微升
价格: 315美元
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抗ERK1 MAPK3兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00104-2
domestic rabbit 单克隆 (ACE-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Immunohistochemical analysis of paraffin-embedded human bladder, using ERK1 Antibody(M00104-2). MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (M00104-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of ERK1 in Jurkat cell lysate (M00104-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
规格: 100微升
价格: 315美元
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