抗CD31 PECAM1抗体
武汉博士德生物工程有限公司
目录: A01513
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

CD31 was detected in paraffin-embedded sections of vascular endothelium in human colon tissues at 20x (B) and at 40x (C) using rabbit anti- CD31 Antigen Affinity purified polyclonal antibody (Catalog # A01513) at 4.0 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

Western blot analysis of CD31 expression in NIH3T3 whole cell lysates (lane 1), JURKAT whole cell lysates (lane 2) and HT-29 whole cell lysates (lane 3). CD31 at 80KD was detected using rabbit anti- CD31 Antigen Affinity purified polyclonal antibody (Catalog # A01513) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100微升
价格: 487美元
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Anti-CD31/PECAM1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9094
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片

Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human CD31 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 39KD. The expected band size for CD31 is at 39KD.

Figure 2. Western blot analysis of CD31 using anti-CD31 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysate,. lane 2: U937 whole cell lysate,. lane 3: MM231 whole cell lysate,. lane 4: JURKAT whole cell lysate,. lane 5: RAJI whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 82KD. The expected band size for CD31 is at 82KD.

Figure 3. IHC analysis of CD31 using anti-CD31 antibody (PB9094). CD31 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD31 Antibody (PB9094) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗CD31/PECAM1抗体
武汉博士德生物工程有限公司
目录: PA1950-1
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化

Anti-CD31 antibody, PA1950-1, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-CD31 antibody, PA1950-1, IHC(P). IHC(P): Human Placenta Tissue

Anti-CD31 antibody, PA1950-1, IHC(F). IHC(F): Human Placenta Tissue
规格: 100μg/vial
价格: 315美元
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抗CD31 PECAM1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01513-1
domestic rabbit 单克隆 (CEE-16)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human kindey, using CD31 Antibody(M01513-1). PECAM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PECAM1 Antibody (M01513-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD31 expression in THP-1 cell lysates (M01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody (Catalog # M01513-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PECAM1
规格: 100微升
价格: 315美元
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Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC01513-FITC
小鼠 单克隆 (JC70)
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪

Monocytes gated PBMCs stained with FITC conjugated anti-human CD31 (clone JC70) (red histogram). Monocytes gated PBMCs stained with FITC conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
规格: 100试验, 200试验
价格: 235美元
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抗CD31 PECAM1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01513-2
domestic rabbit 单克隆 (DAI-16)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 流式细胞仪

Western blot analysis of CD31 expression in THP1 cell lysate (M01513-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody (Catalog # M01513-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PECAM1
规格: 100微升
价格: 315美元
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抗CD31 PECAM1抗体
武汉博士德生物工程有限公司
目录: A01513-4
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 697美元
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抗CD31/PECAM-1(T709)抗体
武汉博士德生物工程有限公司
目录: A01513T709
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 512美元
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抗磷酸化CD31(Y713)PECAM1抗体
武汉博士德生物工程有限公司
目录: A01513Y713
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 380美元
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Anti-Human CD31 PECAM1 Monoclonal Antibody Unconjugated Conjugated, Flow Validat ...
武汉博士德生物工程有限公司
目录: FC01513
小鼠 单克隆 (JC70)
反应物种: 人类
应用: 流式细胞仪
规格: 100微克, 500微克
价格: 147美元
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抗CD31(PECAM-1)兔单克隆抗体, Clone#RM247
武汉博士德生物工程有限公司
目录: M01513-3
domestic rabbit 单克隆 (RM247)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 399美元
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Anti-CD31/PECAM1 Antibody Picoband™ (monoclonal, 2D4)
武汉博士德生物工程有限公司
目录: M01513-4
小鼠 单克隆 (2D4)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片, 免疫组化-冰冻切片
规格: 100µg/vial
价格: 315美元
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