抗孕酮受体/PGR抗体
武汉博士德生物工程有限公司
目录: PA1413
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片
Anti-Progesterone Receptor/PGR Antibody Picoband‚Ñ¢
武汉博士德生物工程有限公司
目录: PB9009
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Figure 1. IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
规格: 10微克样本
价格: 99美元
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抗PR(磷酸化S190)PGR抗体
武汉博士德生物工程有限公司
目录: A00541S190
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫组化
规格: 100微升
价格: 447美元
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抗磷酸化PR(S190)PGR抗体
武汉博士德生物工程有限公司
目录: A00541S190-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格: 100微升
价格: 280美元
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抗PR/PGR(磷酸化S294)抗体
武汉博士德生物工程有限公司
目录: A00541S294
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 447美元
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抗PGR(磷酸化S400)抗体
武汉博士德生物工程有限公司
目录: A00541S400
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 447美元
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小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫组化
规格: 100 ug+FREE HRP Secondary BA1050
价格: 440美元
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抗PR PGR单克隆抗体
武汉博士德生物工程有限公司
目录: M00541-2
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫组化, 免疫细胞化学
规格: 100微升
价格: 280美元
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抗PR(孕激素受体)PGR兔单克隆抗体, Clone#RM357
武汉博士德生物工程有限公司
目录: M00541-3
domestic rabbit 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格:
价格:
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抗磷酸化孕酮受体(Ser190)PGR抗体
武汉博士德生物工程有限公司
目录: P00541
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 435美元
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抗磷酸化孕酮受体(Ser294)PGR抗体
武汉博士德生物工程有限公司
目录: P00541-1
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 435美元
至产商