抗磷脂酰肌醇(-3)激酶p85甲/磷酸肌醇3激酶调节亚单位1抗体
武汉博士德生物工程有限公司
目录: PA1410
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Anti-PI 3 Kinase p85 alpha antibody, PA1410, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: COLO-320 whole cell lysate. Lane 4: SW620 Cell Lysate.

Figure 2. Western blot analysis of PI 3 Kinase p85 alpha using anti-PI 3 Kinase p85 alpha antibody (PA1410). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. Lane 2: rat lung tissue lysates. Lane 3: rat PC-12 whole cell lysates. Lane 4: mouse brain tissue lysates. Lane 5: mouse HEPA1-6 whole cell lysates. Lane 6: mouse RAW246.7 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha antigen affinity purified polyclonal antibody (Catalog # PA1410) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha at approximately 85KD. The expected band size for PI 3 Kinase p85 alpha is at 85KD.
规格: 100μg/vial
价格: 315美元
至产商
Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00318-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100 µg/vial
价格: 315美元
至产商
抗磷脂酰肌醇(-3)激酶p85甲兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00318-2
domestic rabbit 单克隆 (17P38)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
至产商