domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Anti- PKM2 Picoband antibody, PB9379, Western blotting. All lanes: Anti PKM2 (PB9379) at 0.5ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40ug. Lane 2: MM231 Whole Cell Lysate at 40ug. Lane 3: MM453 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 58KD
Anti- PKM2 Picoband antibody, PB9379, IHC(P). IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (GCB-16)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
Western blot analysis of PKM2 expression in HeLa cell lysate (M01173). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM monoclonal antibody (Catalog # M01173) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKM
规格: 100微升
价格: 315美元
至产商
小鼠 单克隆 (11I4C3)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
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