domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹
反应物种: 人类, 小鼠
应用: 免疫印迹
Anti-TPA Picoband antibody, PB9345, Western blotting. All lanes: Anti TPA (PB9345) at 0.5ug/ml. Lane 1: Mouse Lung Tissue Lysate at 50ug. Lane 2: Mouse Testis Tissue Lysate at 50ug. Lane 3: U87 Whole Cell Lysate at 40ug. Lane 4: A431 Whole Cell Lysate at 40ug. Lane 5: A375 Whole Cell Lysate at 40ug. Lane 6: A549 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human U2OS whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: rat liver tissue lysates,. Lane 5: rat kidney tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 55-63KD. The expected band size for PLAT is at 63KD.
Figure 2. IHC analysis of PLAT using anti-PLAT antibody (A02965-1). PLAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PLAT Antibody (A02965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow Cytometry analysis of U251 cells using anti-PLAT antibody (A02965-1). Overlay histogram showing U251 cells stained with A02965-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAT Antibody (A02965-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
规格: 100µg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
domestic rabbit 单克隆 (AOAC-16)
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
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