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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 细胞色素P450还原酶 抗体
抗细胞色素P450还原酶/POR抗体
武汉博士德生物工程有限公司
目录: PA1952
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Anti-POR antibody, PA1952, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Testis Tissue Lysate. Lane 4: Rat Spleen Tissue Lysate. Lane 5: A549 Cell Lysate . Lane 6: HELA Cell Lysate. Lane 7: SKOV Cell Lysate. Lane 8: MCF-7 Cell Lysate
Anti-POR antibody, PA1952, IHC(P). IHC(P): Human Placenta Tissue
Anti-POR antibody, PA1952, ICC. ICC: HELA Cell
规格: 100μg/vial
价格: 315美元
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Anti-Cytochrome P450 Reductase/POR Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9736
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
Figure 1. Western blot analysis of POR using anti-POR antibody (PB9736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate,. Lane 2: Human Placenta Tissue Lysate,. Lane 3: HEPG2 Whole Cell Lysate,. Lane 4: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PB9736) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 85KD. The expected band size for POR is at 77KD.
Figure 2. IHC analysis of POR using anti-POR antibody (PB9736). POR was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of POR using anti-POR antibody (PB9736). POR was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
抗人类POR DyLight系列染料®488共轭标签抗体
武汉博士德生物工程有限公司
目录: A02166-Dyl488
domestic rabbit 多克隆
反应物种:
人类
共轭标签: DyLight488
应用:
流式细胞仪
规格: 50 ug/vial
价格: 415美元
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抗人类POR DyLight系列染料®550共轭标签抗体
武汉博士德生物工程有限公司
目录: A02166-Dyl550
domestic rabbit 多克隆
反应物种:
人类
共轭标签: DyLight系列染料550
应用:
流式细胞仪
Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗细胞色素P450还原酶单克隆抗体
武汉博士德生物工程有限公司
目录: M02166
domestic rabbit 单克隆 (AEFH-16)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
规格: 100微升
价格: 315美元
至产商
Anti-POR Antibody Picoband™ (monoclonal, 7F5)
武汉博士德生物工程有限公司
目录: M02166-2
小鼠 单克隆 (7F5)
反应物种:
人类
应用:
免疫印迹
,
流式细胞仪
,
免疫组化-石蜡切片
规格: 100 µg/vial
价格: 315美元
至产商
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