domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Anti- AMPK beta 2 Picoband antibody, PB9739, Western blotting. All lanes: Anti AMPK beta 2 (PB9739) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug. Lane 3: PANC Whole Cell Lysate at 40ug. Predicted bind size: 30KD. Observed bind size: 30KD
Anti- AMPK beta 2 Picoband antibody, PB9739, IHC(P). IHC(P): Mouse Intestine Tissue
Anti- AMPK beta 2 Picoband antibody, PB9739, IHC(P). IHC(P): Rat Intestine Tissue
规格: 100μg/vial
价格: 315美元
至产商
小鼠 单克隆 (6G1)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
Figure 2. Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (M05077). Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, . Lane 2: human placenta tissue lysate,. Lane 3: human 293T whole cell lysate,. Lane 4: human A549 whole cell lysate,. Lane 5: human A375 whole cell lysate,. Lane 6: human A431 whole cell lysate,. Lane 7: human U20S whole cell lysate,. Lane 8: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AMPK beta 2 antigen affinity purified monoclonal antibody (Catalog # M05077) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
规格: 100µg/vial
价格: 315美元
至产商
小鼠 单克隆 (6G1)
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪
小鼠 单克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪
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