domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Anti-ERK2 antibody, PA1310, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Placenta Tissue Lysate. Lane 4: HT1080 Cell Lysate. Lane 5: HELA Cell Lysate. Lane 6: MM231 Cell Lysate. Lane 7: RAJI Cell Lysate. Lane 8: COLO320 Cell Lysate
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Anti-ERK2 antibody, PA1310-1, All Western blotting. All lanes: Anti-MAPK1(PA1310-1) at 0.5ug/ml. Lane 1: Human Placenta Tissue Lysate at 40ug. Lane 2: Rat Liver Tissue Lysate at 40ug. Lane 3: Rat Brain Tissue Lysate at 40ug. Lane 4: Rat Thymus Tissue Lysate at 40ug. Lane 5: HELA Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Lane 7: COLO320 Whole Cell Lysate at 40ug. Lane 8: MM231 Whole Cell Lysate at 40ug. Lane 9: CEM Whole Cell Lysate at 40ug. Predicted bind size: 41KD. Observed bind size: 41KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (II-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
Figure 2. IHC analysis of MAPK1 using anti-MAPK1 antibody (M00030). MAPK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK1 Antibody (M00030) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of MAPK1 using anti-MAPK1 antibody (M00030). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK1 antigen affinity purified polyclonal antibody (Catalog # M00030) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAPK1.
规格: 100微升
价格: 315美元
至产商
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