抗SHP2/PTPN11抗体
武汉博士德生物工程有限公司
目录: PA1114
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
抗SHP2/PTPN11抗体
武汉博士德生物工程有限公司
目录: PA1860
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Figure 1. Western blot analysis of SHP2 using anti- SHP2 antibody (PA1860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Heart Tissue Lysate,. Lane 2: Rat Skeletal Muscle Tissue Lysate,. Lane 3: Rat Brain Tissue Lysate,. Lane 4: HELA Whole Cell Lysate,. Lane 5: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SHP2 antigen affinity purified polyclonal antibody (Catalog # PA1860) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Figure 2. Western blot analysis of SHP2 using anti- SHP2 antibody (PA1860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,. Lane 2: Rat Kidney Tissue Lysate,. Lane 3: Rat Heart Tissue Lysate,. Lane 4: Rat Skeletal Muscle Tissue Lysate,. Lane 5: A431 Whole Cell Lysate,. Lane 6: JURKAT Whole Cell Lysate,. Lane 7: HELA Whole Cell Lysate,. Lane 8: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SHP2 antigen affinity purified polyclonal antibody (Catalog # PA1860) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.
规格: 100μg/vial
价格: 315美元
至产商
Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6)
武汉博士德生物工程有限公司
目录: M00150-2
小鼠 单克隆 (2000000)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Boster Kit Box
规格: 100µg/vial
价格: 315美元
至产商