抗肌动蛋白ACTA1抗体(单克隆, AC-40)
武汉博士德生物工程有限公司
目录: MA1000
小鼠 单克隆 (AC-40)
反应物种: 人类, 小鼠, 大鼠,
应用: 免疫印迹, 免疫组化
抗肌动蛋白ACTA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M02014-1
domestic rabbit 单克隆 (BE-1)
反应物种: 人类, 小鼠, 大鼠,
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of Actin expression in (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate (M02014-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1

Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody(M02014-1). ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of Hela cells using anti- Actin antibody (M02014-1) . Actin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Actin Antibody (M02014-1) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
至产商
抗肌动蛋白(阿尔法肌动蛋白)ACTA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M02014-4
domestic rabbit 单克隆 (C-1)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪

Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate (M02014-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # M02014-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTA1

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody(M02014-4). ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (M02014-4)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of immunocytochemical section of Hela cells using anti- Actin (Alpha-Actin) antibody (M02014-4) . Actin (Alpha-Actin) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Actin (Alpha-Actin) Antibody (M02014-4) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
至产商
抗肌动蛋白ACTA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M02014-2
domestic rabbit 单克隆 (AAOF-1)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀
规格: 100微升
价格: 315美元
至产商
抗肌动蛋白ACTA1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M02014-3
domestic rabbit 单克隆 (AAOG-1)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 315美元
至产商
Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5)
武汉博士德生物工程有限公司
目录: M02014-5
小鼠 单克隆 (3H5)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商