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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 p65 抗体
抗NF-κB p65/RELA抗体
武汉博士德生物工程有限公司
目录: PA1125
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
Anti-NF-kB p65 antibody, PA1125, Western blotting. All lanes: Anti NF-kB p65 (PA1125) at 0.5ug/ml. Lane 1: Human Colon Cancer Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Predicted bind size: 65KD. Observed bind size: 65KD
规格: 100μg/vial
价格: 315美元
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抗NF-κB p65/RELA抗体
武汉博士德生物工程有限公司
目录: PA1669
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Anti-NF-kB p65 antibody, PA1669, Western blotting. Lane 1: Rat Testis Tissue Lysate . Lane 2: HELA Cell Lysate . Lane 3: A431 Cell Lysate . Lane 4: JURKAT Cell Lysate .
Anti-NF-kB p65 antibody, PA1669, IHC(P). IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
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Anti-NF-kB p65/RELA Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9087
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Anti-NF-kB p65 Picoband antibody, PB9087-1.jpg. All lanes: Anti- NF-kB p65 (PB9087) at 0.5ug/ml. Lane 1: Jurkat Whole Cell Lysate at 40ug. Lane 2: Colo320 Whole Cell Lysate at 40ug. Lane 3: Hela Whole Cell Lysate at 40ug. Predicted bind size: 64 KD. Observed bind size: 64KD
规格: 100μg/vial
价格: 315美元
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抗核因子κB p65 RELA兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00284
domestic rabbit 单克隆 (HH-18)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-kappaB p65 Antibody(M00284). RELA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RELA Antibody (M00284)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of NF-kappaB p65 expression in HeLa cell lysate (M00284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELA monoclonal antibody (Catalog # M00284) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RELA
规格: 100微升
价格: 315美元
至产商
Anti-NF-kB p65/RELA Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00284-1
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Figure 1. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, . Lane 2: rat lung tissue lysates, . Lane 3: rat kidney tissue lysates, . Lane 4: rat testis tissue lysates, . Lane 5: mouse spleen tissue lysates, . Lane 6: mouse lung tissue lysates, . Lane 7: mouse testis tissue lysates, . Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 2. Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,. Lane 2: human A549 whole cell lysates,. Lane 3: human HepG2 whole cell lysates,. Lane 4: human MDA-MB-231 whole cell lysates,. Lane 5: human PANC-1 whole cell lysates,. Lane 6: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
Figure 3. IHC analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). NF-kB p65 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-NF-kB p65 Antibody (A00284-1) overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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抗磷酸化NF B p65(S529)RELA兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00284
domestic rabbit 单克隆 (AbN84)
反应物种:
人类
应用:
免疫印迹
,
免疫沉淀
,
流式细胞仪
Western blot analysis of Phospho-NF-Kappa B p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha (P00284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELA monoclonal antibody (Catalog # P00284) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RELA
规格: 100微升
价格: 315美元
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