Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)
武汉博士德生物工程有限公司
目录: MA1115
小鼠 单克隆 (AC-15)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
文章摘录数: 34

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: Rat Cardiac Muscle Tissue Lysate. Lane 4: Rat Brain Tissue Lysate. Lane 5: A453 Cell Lysate. Lane 6: HE

Anti-beta-Actin antibody (monoclonal), MA1115, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Spleen Tissue Lysate. Lane 3: Rat Brain Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: HELA Cell Lysate. Lane 6: SMMC Cell

Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P). IHC(P): Mouse Intestine Tissue
规格: 100μg/vial
价格: 315美元
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抗beta肌动蛋白ACTB兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01263
domestic rabbit 单克隆 (BF-1)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 1

Immunofluorescent analysis of Hela cells, using beta Actin Antibody

Immunohistochemical analysis of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart, using beta Actin Antibody(M01263). ACTB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTB Antibody (M01263)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate (M01263). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTB monoclonal antibody (Catalog # M01263) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTB
规格: 100微升
价格: 315美元
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抗beta肌动蛋白ACTB抗体
武汉博士德生物工程有限公司
目录: A01263
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化

Western blot analysis of beta Actin expression in MW marker (lane 1), human embryonic kidney 293 cell lysates (lane 2), human lung carcinoma A549 cell lysates (lane 3) and mouse brain extract (lane 4). Load: 35 µg per lane. Beta Actin at 42 kDa (arrowhead) was detected using rabbit anti- beta Actin affinity purified polyclonal antibody (Catalog # A01263) at 1:1,500.

Beta Actin was detected in paraffin-embedded sections of rana pipiens tissues using rabbit anti- beta Actin affinity purified polyclonal antibody (Catalog # A01263) at 1:200. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
规格: 200微克
价格: 404美元
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抗beta肌动蛋白ACTB抗体
武汉博士德生物工程有限公司
目录: A01263-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格: 0.1毫克
价格: 404美元
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抗beta肌动蛋白ACTB抗体
武汉博士德生物工程有限公司
目录: A01263-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 0.1毫克
价格: 404美元
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抗beta肌动蛋白ACTB抗体(生物素)
武汉博士德生物工程有限公司
目录: A01263-Biotin
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
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抗beta肌动蛋白ACTB抗体(HRP)
武汉博士德生物工程有限公司
目录: A01263-HRP
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
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抗beta肌动蛋白小鼠单克隆抗体(HRP共轭标签)
武汉博士德生物工程有限公司
目录: H01263
小鼠 单克隆 (17A12)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
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Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112
武汉博士德生物工程有限公司
目录: M01263-2
domestic rabbit 单克隆 (RM112)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀
规格: 100微升
价格: 399美元
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抗beta肌动蛋白抗体
武汉博士德生物工程有限公司
目录: M01263-3
小鼠 单克隆 (monoclonal)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 80微升
价格: 173美元
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抗beta肌动蛋白小鼠单克隆抗体
武汉博士德生物工程有限公司
目录: M01263-4
小鼠 单克隆 (17A10)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100 µl/vial
价格: 315美元
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抗beta肌动蛋白小鼠单克隆抗体
武汉博士德生物工程有限公司
目录: M01263-5
小鼠 单克隆 (17A11)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
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抗beta肌动蛋白ACTB单克隆抗体[10B7](生物素)
武汉博士德生物工程有限公司
目录: M01263-Biotin
小鼠 单克隆 (10B7)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
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