抗SHC/SHC1抗体
武汉博士德生物工程有限公司
目录: PA1897
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Anti-SHC antibody, PA1897, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578). Lane 1: Recombinant Human SHC1 Proteins 10ng. Lane 2: Recombinant Human SHC1 Proteins 5ng. Lane 3: Recombinant Human SHC1 Proteins 2.5ng

Figure 2. Western blot analysis of SHC using anti-SHC antibody (PA1897). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, . Lane 2: human Hela whole cell lysate, . Lane 3: human HepG2 whole cell lysate, . Lane 4: human Jurkat whole cell lysate, . Lane 5: rat C6 whole cell lysate, . Lane 6: mouse thymus tissue lysate, . Lane 7: mouse RAW246.7 whole cell lysate, . Lane 8: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PA1897) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.

Anti-SHC antibody, PA1897, IHC(P). IHC(P): Rat Brain Tissue
规格: 100μg/vial
价格: 315美元
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Anti-SHC/SHC1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9391
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Figure 1. Western blot analysis of SHC using anti-SHC antibody (PB9391). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, . Lane 2: human Hela whole cell lysate, . Lane 3: human HepG2 whole cell lysate, . Lane 4: human Jurkat whole cell lysate, . Lane 5: human K562 whole cell lysate, . Lane 6: human THP-1 whole cell lysate, . Lane 7: rat C6 whole cell lysate, . Lane 8: mouse thymus tissue lysate, . Lane 9: mouse RAW246.7 whole cell lysate, . Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PB9391) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.

Anti- SHC1 Picoband antibody, PB9391, IHC(P). IHC(P): Mouse Brain Tissue

Anti- SHC1 Picoband antibody, PB9391, IHC(P). IHC(P): Rat Brain Tissue
规格: 100μg/vial
价格: 315美元
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抗SHC单克隆抗体
武汉博士德生物工程有限公司
目录: M00796-1
domestic rabbit 单克隆 (ADFI-19)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 315美元
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Anti-SHC Antibody Picoband™ (monoclonal, 6D6E1)
武汉博士德生物工程有限公司
目录: M00796-3
小鼠 单克隆 (6D6E1)
反应物种: 人类
应用: 免疫印迹
规格: 100 µg/vial
价格: 315美元
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