抗GLUT1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00163
兔 单克隆 (CGG-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Immunofluorescent analysis of HepG2 cells, using GLUT1 Antibody .

Western blot analysis of GLUT1 expression in HepG2 lysate (M00163). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 monoclonal antibody (Catalog # M00163) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1

Immunohistochemical analysis of paraffin-embedded human cervix cancer, using GLUT1 Antibody(M00163). SLC2A1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC2A1 Antibody (M00163)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100 ug/vial
价格: 299美元
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Anti-SLC2A1 Picoband Antibody (monoclonal, 10C10)
武汉博士德生物工程有限公司
目录: M00163-1
小鼠 单克隆 (10C10)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片

Boster Kit Box
规格: 100 ug/vial
价格: 280美元
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