抗5-羟色胺转运蛋白/SLC6A4抗体
武汉博士德生物工程有限公司
目录: PA1705
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-SLC6A4 antibody, PA1705, Western blotting. All lanes: Anti SLC6A4 (PA1705) at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 70KD. Observed bind size: 70KD
规格: 100μg/vial
价格: 315美元
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Anti-Serotonin transporter/SLC6A4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9438
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG whole cell lysates, . Lane 2: human Caco-2 whole cell lysates, . Lane 3: human SW620 whole cell lysates, . Lane 4: human COLO-320 whole cell lysates. Lane 5: human K562 whole cell lysates, . Lane 6: human U2OS whole cell lysates, . Lane 7: human U-87MG whole cell lysates, . Lane 8: rat heart tissue lysates, . Lane 9: mouse heart tissue lysates, . Lane 10: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (Catalog # PB9438) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 90KD. The expected band size for SLC6A4 is at 70KD.

Figure 2. IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). SLC6A4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). SLC6A4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
抗SLC6A4抗体
武汉博士德生物工程有限公司
目录: A00496
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 637美元
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抗SLC6A4抗体
武汉博士德生物工程有限公司
目录: A00496-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 380美元
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抗5-羟色胺转运蛋白兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00496
domestic rabbit 单克隆 (18S75)
反应物种: 人类
应用: 免疫印迹
规格: 100 µl/vial
价格: 315美元
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抗磷酸化5-羟色胺转运蛋白(Thr276)SLC6A4抗体
武汉博士德生物工程有限公司
目录: P00496
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100µl
价格: 435美元
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