domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化
反应物种: 人类
应用: 免疫印迹, 免疫组化
Anti-SPARC antibody, PA1585, Western blotting. Lane 1: Recombinant Human SPARC Protein 10ng. Lane 2: Recombinant Human SPARC Protein 5ng. Lane 3: Recombinant Human SPARC Protein 2.5ng
Anti-SPARC antibody, PA1585, Western blotting. WB: HELA Cell Lysate
Anti-SPARC antibody, PA1585, IHC(P). IHC(P): Human Intestinal Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: rat testis tissue lysates, . Lane 3: mouse brain tissue lysates, . Lane 4: mouse testis tissue lysates, . Lane 5: U20s whole Cell lysates, . Lane 6: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 43KD. The expected band size for SPARC is at 35 KD.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates,. Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 37KD. The expected band size for SPARC is at 35KD.
规格: 100µg/vial
价格: 315美元
至产商
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