domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
Anti-Src Picoband antibody, PB9059-1.JPG. IHC(P): Human Mammary Cancer Tissue
Anti-Src Picoband antibody, PB9059-2.JPG. IHC(P): Human Intestinal Cancer Tissue
Anti-Src Picoband antibody, PB9059-3.jpg. All lanes: Anti-Src(PB9059) at 0.5ug/ml. Lane 1: COLO320 Whole Cell Lysate at 40ug. Lane 2: A549 Whole Cell Lysate at 40ug. Predicted bind size: 60KD. Observed bind size: 60KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 单克隆 (DCI-19)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Western blot analysis of Src expression in U87-MG cell lysate (M00107-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC monoclonal antibody (Catalog # M00107-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRC
规格: 100微升
价格: 315美元
至产商
domestic rabbit 单克隆 (DDH-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪
Figure 1. Western blot analysis of SRC using anti-SRC antibody (MP00107) in A431 cell lysate treated with pervanadate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # MP00107) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SRC.
规格: 100微升
价格: 315美元
至产商
domestic rabbit 单克隆 (III-19)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
微博分享
关注我们的微博
- 来邦网
- 英文来邦