Anti-BRCA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00005
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Figure 1. Western blot analysis of BRCA1 using anti- BRCA1 antibody (A00005). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, . Lane 2: SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 207KD. The expected band size for BRCA1 is at 207KD.
规格: 100μg/vial
价格: 315美元
至产商
Anti-BRCA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9015
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Anti-BRCA1 Picoband antibody, PB9015-1.JPG. IHC(P): Mouse Testis Tissue

Anti-BRCA1 Picoband antibody, PB9015-2.JPG. IHC(P): Rat Testis Tissue

Anti-BRCA1 Picoband antibody, PB9015-3.JPG. IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
抗乳腺癌1号基因(Ab-1524)抗体
武汉博士德生物工程有限公司
目录: A00005-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 299美元
至产商
Anti-BRCA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00005-2
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
抗磷酸化乳腺癌1号基因(S1457)抗体
武汉博士德生物工程有限公司
目录: A00005S1457
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 380美元
至产商
抗乳腺癌1号基因(磷酸化S1524)抗体
武汉博士德生物工程有限公司
目录: A00005S1524
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 547美元
至产商
Anti-BRCA1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00005-3
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪

Figure 1. Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 290 kDa.
规格: 100 µg/vial
价格: 315美元
至产商