Anti-B Raf/BRAF Antibody
武汉博士德生物工程有限公司
目录: PA1848
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Anti-B Raf antibody, PA1848, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: Rat Liver Tissue Lysate. Lane 4: SW620 Cell Lysate. Lane 5: COLO320 Cell Lysate. Lane 6: PC-12 Cell Lysate.
规格: 100μg/vial
价格: 315美元
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Anti-B Raf/BRAF Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9164
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片

All lanes: Anti B Raf (PB9164) at 0.5ug/ml. WB: Recombinant Human B Raf Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD

All lanes: Anti B Raf (PB9164) at 0.5ug/ml. Lane 1: Rat Testis Tissue at 50ug. Lane 2: Rat Brain Tissue at 50ug. Lane 3: Mouse Testis Tissue at 50ug. Lane 4: Mouse Brain Tissue at 50ug. Lane 5: Hela Whole Cell Lysate at 40ug. Lane 6: Jurkat Whole Cell Lysate at 40ug. Lane 7: MCF-7 Whole Cell Lysate at 40ug. Lane 8: K562 Whole Cell Lysate at 40ug. Predicted bind size: 84KD . Observed bind size: 84KD
规格: 100μg/vial
价格: 315美元
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Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody
武汉博士德生物工程有限公司
目录: P00075
domestic rabbit 单克隆 (IBE-2)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀

Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA (P00075). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRAF monoclonal antibody (Catalog # P00075) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRAF
规格: 100微升
价格: 315美元
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Anti-B Raf Rabbit Monoclonal Antibody
武汉博士德生物工程有限公司
目录: M00075
domestic rabbit 单克隆 (CCG-2)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody(M00075). BRAF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRAF Antibody (M00075)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of B Raf expression in (1)Human brain lysate (2)HepG2 cell lysate (M00075). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRAF monoclonal antibody (Catalog # M00075) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRAF
规格: 100微升
价格: 315美元
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