domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Anti- TLR1 antibody, PA2246, Western blotting. All lanes: Anti TLR1 (PA2246) at 0.5ug/ml. Lane 1: COLO320 Whole Cell Lysate at 40ug. Lane 2: SW620 Whole Cell Lysate at 40ug. Lane 3: SKOV Whole Cell Lysate at 40ug. Lane 4: JURKAT Whole Cell Lysate at 40ug. Lane 5: CEM Whole Cell Lysate at 40ug. Lane 6: PANC Whole Cell Lysate at 40ug. Predicted bind size: 90KD. Observed bind size: 90KD
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
Figure 1. Western blot analysis of TLR1 using anti-TLR1 antibody (A00429-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates,. Lane 2: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody (Catalog # A00429-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD.
Figure 2. IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2). TLR1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TLR1 Antibody (A00429-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2). TLR1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TLR1 Antibody (A00429-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
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