抗肿瘤坏死因子甲(肿瘤坏死因子α)单克隆抗体
武汉博士德生物工程有限公司
目录: M00002
小鼠 单克隆 (TNF706 + P/T2)
反应物种: 人类, 小鼠, 大鼠, 犬
应用: 免疫组化

Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained withTNF alpha Monoclonal Antibody (TNF706 + P/T2).
规格: 100 ug/vial
价格: 440美元
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抗肿瘤坏死因子甲抗体
武汉博士德生物工程有限公司
目录: PA1079
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human placenta tissue lysates, . Lane 3: human U-937 whole cell lysates, . Lane 4: human HL-60 whole cell lysates. Lane 5: human A431 whole cell lysates, . Lane 6: human A549 whole cell lysates, . Lane 7: human K562 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 28KD. The expected band size for TNF alpha is at 25KD.

Figure 2. IHC analysis of TNF alpha using anti-TNF alpha antibody (PA1079). TNF alpha was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF alpha Antibody (PA1079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of TNF alpha using anti-TNF alpha antibody (PA1079). TNF alpha was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNF alpha Antibody (PA1079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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Anti-TNF alpha Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00002-3
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
规格: 100µg/vial
价格: 315美元
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抗肿瘤坏死因子甲(Q178)抗体
武汉博士德生物工程有限公司
目录: A00002
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 512美元
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抗肿瘤坏死因子甲纯化无叠氮物单克隆抗体
武汉博士德生物工程有限公司
目录: M00002-1
小鼠 单克隆 (MAb1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克(C100)
价格: 280美元
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抗肿瘤坏死因子甲纯化单克隆抗体
武汉博士德生物工程有限公司
目录: M00002-2
小鼠 单克隆 (MAb11)
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 0.1毫克(C100)
价格: 280美元
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抗肿瘤坏死因子甲单克隆抗体
武汉博士德生物工程有限公司
目录: M00002-3
小鼠 单克隆 (Q34)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 380美元
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